Journal Article
Microbiome differences between river-dwelling and caveadapted populations of the fish Astyanax mexicanus (De Filippi, 1853)
Ornelas-García, P., Pajares, S., Sosa-Jiménez, V.M., Rétaux, S. and Miranda-Gamboa, R.A.
Record Number:
4066
Year:
2018
Journal:
PeerJ Preprints
Volume:
27058v1
Abstract:
Symbiotic relationships between host and microbiome can play a major role in local
adaptation. Previous studies with freshwater organisms have shown that microbiome
performs numerous important biochemical functions for the host, playing a key role in
metabolism, physiology or health. Experimental studies in fish groups have found an effect
of enzymatic activity of gut microbiota on a variety of metabolic processes. The goal of this
study was to compare stomach microbiome from cave and surface Astyanax mexicanus, in
order to evaluate the potential response of microbiota to locally contrasting environmental
conditions and physiological adaptations of the host. Stomach biopsies were obtained from
three different populations: Pachón cave, and two surface rivers (Rascón and Micos rivers).
The stomach microbiome was analyzed using the Ion 16S Metagenomic kit considering
seven variable regions: V2, V3, V4, V6-7, V8 and V9. A high diversity was observed across
samples, including 16 phyla, 120 families and 178 genera. Gammaproteobacteria,
Firmicutes, Bacteroidetes and Betaprotobacteria were the most abundant phyla across the
samples. Although the relative abundance of the core OTUs at genus level were highly
contrasting among populations, we did not recover differences in stomach microbiome
between contrasting habitats (caves vs surface rivers). Rather, we observed a consistent
association between β-diversity and dissolved oxygen concentration in water. Therefore,
and unexpectedly, the microbiota of A. mexicanus is not linked with the habitat but results
from water conditions.
Times Cited:
1
Relevent Species: