Journal Article
Molecular discrimination of Ancistrus lineages (Siluriformes: Loricariidae) using barcode DNA tool
Splendore de Borba, R., Mariotto, S., Centofante, L., Zawadzki C.H. and Parise-Maltempi, P.P.
Record Number:
4337
Year:
2019
Journal:
Mitochondrial DNA Part A
Abstract:
Although several species of Ancistrus have been described from the Amazon and Paraguay river basins
in the states of Amazonas and Mato Grosso, Brazil, the taxonomic status of most specimens from these
regions remains doubtful. In the present work, cytogenetic and molecular data were used to discriminate
and isolate unexpected Ancistrus lineages from the Amazon and Paraguay basins. For that, it was
used DNA barcoding based on mitochondrial Cytochrome Oxidase Subunit I (COI) gene and cytogenic
data to perform such molecular discrimination. The analyzed sequences had 669 bp, of which 171 bp
were conserved and 491 bp were variable. The Neighbor-joining and Bayesian analysis revealed 21 distinct
groups in topology. The genetic distances within each group was 0.4%, 21 times smaller than the
mean distance observed among groups, which was 8.4%. These values showed seven distinct lineages
of Ancistrus from the studied points of the Amazon basin and eight lineages from the Paraguay basin
points. Our results illustrate the efficiency of this technique for the discrimination of the Ancistrus lineages
once it indicates the occurrence of cryptic species in these regions, which cannot yet be identified
either with just chromosomal or morphological analyzes.
Times Cited:
4