Journal Article
Manipulation of gene function in Mexican cavefish
Stahl, B.A., Jaggard, J.B., Chin, J.S., Kowalko, J.E., Keene, A.C. and Duboué, E.R.
Record Number:
4342
Year:
2019
Journal:
Journal of Visualized Experiments
Pages:
1-12
Volume:
146 e59093
PMCID:
Abstract:
We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.
Times Cited:
1
Relevent Species: