Journal Article
Detection of changes in mitochondrial hydrogen sulfide in vivo in the fish model Poecilia mexicana (Poeciliidae)
Lau, G.Y., Barts, N., Hartley, R.C., Tobler, M., Richards, J.G., Murphy, M.P. and Arndt, S.
Record Number:
4352
Year:
2019
Journal:
Biology Open
Volume:
8, bio041467
Abstract:
In this paper, we outline the use of a mitochondria-targeted ratiometric
mass spectrometry probe, MitoA, to detect in vivo changes in
mitochondrial hydrogen sulfide (H2S) in Poecilia mexicana (family
Poeciliidae). MitoA is introduced via intraperitoneal injection into the
animal and is taken up by mitochondria, where it reacts with H2S to
form the product MitoN. The MitoN/MitoA ratio can be used to assess
relative changes in the amounts of mitochondrial H2S produced over
time. We describe the use of MitoA in the fish species P. mexicana to
illustrate the steps for adopting the use of MitoA in a new organism,
including extraction and purification of MitoA and MitoN from tissues
followed by tandem mass spectrometry. In this proof-of-concept study
we exposed H2S tolerant P. mexicana to 59 μM free H2S for 5 h,which
resulted in increased MitoN/MitoA in brain and gills, but not in liver or
muscle, demonstrating increased mitochondrial H2S levels in select
tissues following whole-animal H2S exposure. This is the first time that
accumulation of H2S has been observed in vivo during whole-animal
exposure to free H2S using MitoA
Times Cited:
1
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