Journal Article
Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation
Krishnan, J., Wang, Y., Kenzior, O., Huzaifa, H., Olsen, L., Tsuchiya, D., Wang, Y., Zhao, C. and Rohner, N.
Record Number:
5650
Year:
2022
Journal:
bioRxiv preprint January 6th 2022
Pages:
1-13
Abstract:
Cell lines have become an integral resource and tool for conducting biological experiments ever since
the Hela cell line was first developed (1). They not only allow detailed investigation of molecular
pathways but are faster and more cost-effective than most in vivo approaches. The last decade saw
many emerging model systems strengthening basic science research. However, lack of genetic and
molecular tools in these newer systems pose many obstacles. Astyanax mexicanus is proving to be an
interesting new model system for understanding metabolic adaptation. To further enhance the utility of
this system, we developed liver-derived cell lines from both surface-dwelling and cave-dwelling
morphotypes. In this study, we provide detailed methodology of the derivation process along with a
comprehensive biochemical and molecular characterization of the cell lines, which reflects key metabolic
traits of cavefish adaptation. We anticipate these cell lines to become a useful resource for the Astyanax
community as well as researchers investigating fish biology, comparative physiology, and metabolism.
Times Cited:
1
Relevent Species: