Journal Article
Revealing the satellite DNA history in Psalidodon and Astyanax Characid fish by comparative satellitomics
Goes, C.A.G., dos Santos, R.Z., Aguiar, W.R.C., Alves, D.C.V., Silva, D.M.Z.dA., Foresti, F., Oliveira, C., Utsunomia, R. and Porto-Foresti, F.
Record Number:
5906
Year:
2022
Journal:
Frontiers in Genetics
Pages:
1-22
Volume:
13:884072
Abstract:
Eukaryotic genomes are usually enriched in repetitive DNA sequences, which can be
classified as dispersed or tandemly repeated elements. Satellite DNAs are noncoding
monomeric sequences organized in a head-to-tail fashion that are generally located on the
subtelomeric and/or pericentromeric heterochromatin. In general, a single species
incorporates a diverse group of satellite DNA families, which collection is called
satellitome. Here, we characterized three new satellitomes from distinct characid fish
(Psalidodon fasciatus, P. bockmanni, and Astyanax lacustris) using a combination of
genomic, cytogenetic, and bioinformatic protocols. We also compared our data with the
available satellitome of P. paranae. We described 57 satellite DNA (satDNA) families of P.
fasciatus (80 variants), 50 of P. bockmanni (77 variants), and 33 of A. lacustris (54 variants).
Our analyses demonstrated that several sequences were shared among the analyzed
species, while some were restricted to two or three species. In total, we isolated 104
distinctive satDNA families present in the four species, of which 10 were shared among all
four. Chromosome mapping revealed that the clustered satDNA was mainly located in the
subtelomeric and pericentromeric areas. Although all Psalidodon species demonstrated
the same pattern of clusterization of satDNA, the number of clusters per genome was
variable, indicating a high dynamism of these sequences. In addition, our results expand
the knowledge of the As51 satellite DNA family, revealing that P. bockmanni and P.
paranae exhibited an abundant variant of 39 bp, while P. fasciatus showed a variant of
43 bp. The majority of satDNAs in the satellitomes analyzed here presented a common
library repetitive sequence in Psalidodon and Astyanax, with abundance variations in each
species, as expected for closely related groups. In addition, we concluded that the most
abundant satDNA in Psalidodon (As51) passed through a diversification process in this
group, resulting in new variants exclusive of Psalidodon.
Keywords: satellitome, satDNA evolution, characiforms, fish, neotropical fishes
Times Cited:
1
Relevent Species: