Journal Article
The preservation of PPARg genome duplicates in some Teleost tineages: Insights into lipid metabolism and xenobiotic exploitation
Páscoa, I., Fonseca, E., Ferraz, R., Machado, A.M., Conrado, F., Ruivo, R., Cunha, I. and Castro, L.F.C.
Record Number:
5995
Year:
2022
Journal:
Genes
Pages:
1-15
Volume:
13, 107
Notes:
Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons
Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Abstract:
Abstract: Three peroxisome proliferator-activated receptor paralogues (PPARa, -b and -g) are currently
recognized in vertebrate genomes. PPAR
is known to modulate nutrition, adipogenesis and
immunity in vertebrates. Natural ligands of PPAR
have been proposed; however, the receptor also
binds synthetic ligands such as endocrine disruptors. Two paralogues of PPARa and PPARb have
been documented in teleost species, a consequence of the 3R WGD. Recently, two PPARg paralogue
genes were also identified in Astyanax mexicanus. We aimed to determine whether the presence of two
PPARg paralogues is prevalent in other teleost genomes, through genomic and phylogenetic analysis.
Our results showed that besides Characiformes, two PPARg paralogous genes were also identified
in other teleost taxa, coinciding with the teleost-specific, whole-genome duplication and with the
retention of both genes prior to the separation of the Clupeocephala. To functionally characterize
these genes, we used the European sardine (Sardina pilchardus) as a model. PPARgA and PPARgB
display a different tissue distribution, despite the similarity of their functional profiles: they are unresponsive
to tested fatty acids and other human PPAR
ligands yet yield a transcriptional response in
the presence of tributyltin (TBT). This observation puts forward the relevance of comparative analysis
to decipher alternative binding architectures and broadens the disruptive potential of man-made
chemicals for aquatic species.
Keywords: PPARg; genome duplication; Clupeocephala; sardine; organotins
Times Cited:
1
Relevent Species: