Journal Article

A protocol for whole-brain Ca2+ imaging in Astyanax mexicanus, a model of comparative evolution

Lloyd, E., Privat, M., Sumbre, G., Duboue, E.R. and Keene, A.C.

Record Number:
6278
Year:
2023
Journal:
Star Protocols
Pages:
1-12
Volume:
4, 102517
PMCID:
https://doi.org/10.1016/j.xpro.2023.102517
Abstract:
In this protocol, we describe a comparative approach to study the evolution of brain function in theMexican tetra, Astyanax mexicanus. We developed surface fish and two independent populations of cavefish with pan-neuronal expression of the Ca2+ sensor GCaMP6s. We describe a methodology to prepare samples and image activity across the optic tectum and olfactory bulb
Times Cited:
1
Relevent Species:
Astyanax jordani (Hubbs and Innes 1936) Buckup 2003